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0032 (0)16 41 44 07 |
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Protéin A
Product Name |
Recombinant A |
Price |
150 € /
500ul |
Source
Sequence |
E coli
Amino
acid 32 -327 of the Staphylococcus aureus subsp aureus . Protein A lg
binding domains with 6X Hist-tag on N-terminus . Gene Bank Accession
Number YP_498670 |
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Purity |
>99 % by
SDS – PAGE and HPLC analyses . Endotexin level is < 0.1 EU /mg of
Protein A |
Mol Weight |
39.2 Kda
|
Form |
Lyophilized |
Raised in |
Rabbit |
Clonality |
Polyclonal |
Form |
Crude
serum |
Storage instructions |
Aliquot
and store at -20°C. Avoid repeated freeze / thaw cycles. |
Expiration |
20/04/2016 |
Application :
The recombinant Protein A is a genetically engineerd
protein containing five lg- binding regions of protein A and 6X Hist – tag on N
terminus . Cell wall binding region and other non specific binding region and
other non specific binding regions have been eliminated from the recombinant
Protein A to ensure the maximum specific lgG binding . The 6 X His- tag on N
terminus can be used for affinity purification or for protein A detection
using anti Hist- tag antibody .
The recombinant Protein A is deal for purification of
polyclonal or monoclonal lgG antibodies .
Protein A binds to most human and mouse lgG subclasses ( eg
human , lgG1 , lgG2, lgG4; mouse lgG1, lgG2a, lgG2b, lgG3 ) It also binds to
total lgG from cow , guinea pig
Usage
: For Research Purpose Only . Not to be used in Human
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