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KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic


 Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy
 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                    Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494 

Ireland Dublin
+35316526556 

Luxembourg
+35220880274 

Magyarország Budapest
+3619980547 

Nederland
+31208080893 

Norge Oslo
+4721031366 

Österreich
+43720880899 

Sverige Stockholm
+46852503438 

Schweiz Züri
+41435006251 

 

Northern America 

Canada Montreal
+15149077481 

US New York
+17185132983

 

Other Countries redcoon België
 0032 (0)16 41 44 07

 
Human TNF (Tumor Necrosis Factor) alpha ELISA Kit
The Exalpha Biologicals, Inc. human Tumor Necrosis Factor alpha (TNFa) ELISA is a non-isotopic immunoassay for the in vitro quantitation of human phospho TNFa in serum, plasma, cell culture supernatants.

 

Features
- Colorimetric assay
- HTS compatible format
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 5 hour protocol

 		 Performance Summary
- Sensitivity: 20 pg/ml
- Precision: <10% well-to-well variation
- Linearity: Within 5% of expected values
Ordering Information
Cat. No.
X1851K (pdf)
 		 Size
1 Plate
 
For larger quantity pricing, please contact Customer Service

Protocol Summary: Not to be used in place of detailed protocol. See kit manual for complete protocol.

Steps
Incubation
1. Equilibrate kit to room temperature -
2. Add samples and standards to wells 2 hours room temp. (or overnight 4°C)
3. Wash 4x -
4. Add detector antibody to all wells 2 hours - room temp.
5. Wash 4x -
6. Add HRP conjugate to all wells 30 minutes - room temp.
7. Wash 4x -
8. Add substrate to all wells 30 minutes - room temp.
9. Add stop solution to all wells -
10. Read plate at 450 nm/550 nm -