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OUR SUPPLIERS
KOMABIOTECH
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Spherotech, Inc.
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Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
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Laboratories, Inc. Headquarters
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Frontier™ PIG-3 (Human p53 Inducible Gene
3), A Stable Marker for p53 Induction, ELISA Detection Kit
(Patent Pending) |
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Exalpha’s Frontier™ PIG3 Assay is a
non-isotopic, non-radioactive immunoassay for the
quantitation of the p53 inducible gene 3 (PIG-3) - a
long-lived reporter of transient p53 activation.1
The assay is suitable for human sera, plasma, tissue culture
supernatants and cellular extracts or lysates.
1. Flatt, P.M.
"p53-dependent expression of PIG3 during proliferation,
genotoxic stress, and reversible growth arrest" Cancer Lett
2000, 156, 63-72
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Features
- High sensitivity
- 4 deg C storage with long shelf life
- Non-radioactive
- 2.5 hour protocol
- Suitable for sera, plasma, culture supernatants, cellular
extracts/lysates
- HTS compatible format |
Performance Summary
- Sensitivity: 40 pg/ml
- Recovery: serum, >98%; plasma, >98%
Precision Studies
- Intra-assay C.V.'s: <3% mid-level
- Inter-assay C.V.'s: <4% mid-level |
| Ordering Information |
Cat. No.
X1326K1
X1326K2
X1326K3 |
Size
1 x 96 wells
2 x 96 wells
10 x 96 wells |
| For larger quantity pricing, please
contact Customer Service |
 |
Legend:
Exalpha’s Frontier™ PIG-3 assay. Graphs show pooled coefficients
of variation and between assay coefficients of variation (top),
senitivity of assay with ng/ml of PIG-3 versus absorbance at
450-550 nm (middle) and standard curve with ng/ml of PIG-3
versus absorbance at 450-550 nm (bottom). |
Protocol Summary: Not to be used in place of
detailed protocol. See kit manual for complete protocol.
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1. Equilibrate kit to room temperature |
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2. Add samples and
standards to wells
Add detector antibody to wells
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• Incubate 2 hours at
room temperature |
| 3. Wash 3 times |
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| 4. Add conjugate to all
wells |
• Incubate 30 minutes
at room temperature |
| 5. Wash 3 times |
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| 6. Add substrate to all
wells |
• Incubate 30 minutes
at room temperature |
| 7. Add stop solution to
all wells |
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| 8. Read plate(s) at 450
nm/550 nm |
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