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KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic


 Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy
 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                    Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719 

Danmark

+4569918806 

Finland Helsset
+358942419041

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+302111768494 

Ireland Dublin
+35316526556 

Luxembourg
+35220880274 

Magyarország Budapest
+3619980547 

Nederland
+31208080893 

Norge Oslo
+4721031366 

Österreich
+43720880899 

Sverige Stockholm
+46852503438 

Schweiz Züri
+41435006251 

 

Northern America 

Canada Montreal
+15149077481 

US New York
+17185132983

 

Other Countries redcoon België
 0032 (0)16 41 44 07

 
APO ACTIVE 3 ™
Antibody Specific Active Caspase 3 Detection Kit
 
Key Benefits:
  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications  - Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
Assay Principle
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Kit contents
  • 1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody
  • 1 vial of FITC labeled Goat anti Rabbit affinity purified polyclonal antibody
  • 1 vial of 10X fixative.
The following kits are available:
Product
 		 Catalog No.
 		 Size
(No. of Tests)		 Price (Eur)
APO ACTIVE 3 Antibody Specific Active Caspase 3 Detection Kit FAB200-1 25 €145
FAB200-2
 		 100 €425

(A)

(B)
Jurkat Cells were stimulated with staurosporine (B) or DMSO (A) for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.