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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
Marienbongard 20
52074 Aachen,
Germany
Tel 0241 56 00
99 68
Fax 0241 56 00 47 88
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 58 760 77
08
Fax: 00 32 16 50 90
45
GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
93
Fax 02 36 00 65
94
Česká republika
Praha
+420246019719
Danmark
+4569918806
Finland Helsset
+358942419041
Ελλάς Αθήνα
+302111768494
Ireland Dublin
+35316526556
Luxembourg
+35220880274
Magyarország
Budapest
+3619980547
Nederland
+31208080893
 Norge
Oslo
+4721031366
Österreich
+43720880899
 Sverige
Stockholm
+46852503438
Schweiz Züri
+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
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| Block Buffers |
| Improves Assays: |
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Increases the Sensitivity of the Assay. This is
done by stabilizing the antigenic and functional regions of the
absorbed protein.
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Reduces Background Noise. Block buffers inhibit
nonspecific binding to uncoated regions of the plate and
non-specific binding sections of the absorbed proteins. This
leads to lower background noise and high specific signal.
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Increases Efficiency and Extends the Shelf-Life of the
Coated Plates. With a longer shelf-life, plates may be
prepared in large batches to be used over time. Large batch
sizes will increase efficiency and reduce plate-to-plate
variability to increase consistency.
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Conserves Valuable Reagents. By promoting a
higher specific signal, less protein may be needed to coat the
plate and less of the detection molecules may be needed to
generate a signal when the proper blocker is used.
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| Blockers Available: |
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EBB100 General-Purpose Blocker with BSA-Based Blockers
EBB200 Non-Mammalian-Based Blocker
EBB300 Synthetic Blocker with Tween
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| Directions: |
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Once your plate has been coated with an antibody or antigen,
aspirate or dump out the coating solution. Then, simply pipette
300-400uL per well of block buffer and let it incubate (Cell
Technology recommends a minimum incubation of 3 hours).
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| EBB100 General-Purpose
Blocker with BSA-Based Blockers |
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General-Purpose Blocker contains a mammalian protein blocking agent
(based on BSA) suitable for most monoclonal and polyclonal antibody
capture ELISA formats, as well as peptide and protein antigen-down
ELISA formats. This unique block buffer contains proprietary protein
stabilizers that provide a long-term stabilizing environment for
dried antigen or antibody coat proteins, while minimizing
nonspecific binding interactions during the assay process.
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| Catalog Number |
Size |
#Plates at 300uL/well
(28.8mL/plate)
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#Plates at 400uL/well
(38.4mL/plate) |
Price |
| EBB100-1 |
100 |
3 |
2 |
€45.00 |
| EBB100-2 |
500
|
16 |
12 |
€120.00 |
| EBB100-3 |
1,000 |
33 |
25 |
€199.00 |
| EBB100-4 |
10,000 |
330 |
250 |
€1900.00 |
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| EBB200 Nonmammalian-Based Blocker |
Cell Technology's Nonmammalian-Based Blocker contains a proprietary
non-mammalian formulation based on fish proteins, plus molecular
stabilizers that provide a high degree of blocking efficiency.
EBB200 was designed for antigen-down ELISA formats, and for sandwich
assays with high background problems. This formulation is a
heterogeneous mixture of small molecules capable of blocking both
the nonspecific binding sites of the absorbed protein, as well as
the unoccupied regions of the polystyrene plate that are not
sterically accessible to larger traditional mammalian blocking
reagents, thereby reducing background noise. The small size of these
unique blockers results in minimal steric hindrance to key epitope
regions of target coated proteins, thus enhancing specific signal
generation.
EBB200 reduces the possibility of false-positives generated from
endogenous antibodies in the sample reacting with plate blocking
proteins. It is particularly useful when working with human, swine,
and bovine serum samples, as there is little interaction with the
blocking molecules resulting in lower backgrounds. |
| Catalog Number |
Size |
#Plates at 300uL/well
(28.8mL/plate)
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#Plates at 400uL/well
(38.4mL/plate) |
Price |
| EBB200-1 |
100 |
3 |
2 |
€45.00 |
| EBB200-2 |
500
|
16 |
12 |
€140.00 |
| EBB200-3 |
1,000 |
33 |
25 |
€270.00 |
| EBB200-4 |
10,000 |
330 |
250 |
€2500.00 |
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| EBB300 Synthetic Blocker |
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Cell Technology's Synthetic Blocker is a novel non-protein blocking
formulation designed to eliminate interference and nonspecific
background noise associated with antibody-coated ELISA formats and
sandwich ELISAs. Because Synthetic Blocker contains inert
non-reactive blockers, it reduces the amount of nonspecific binding
of enzyme-labeled conjugates to blocked plate surfaces. Use of this
blocking formulation provides superior background performance
without the use of conventional cross-reactive protein additives.
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| Catalog Number |
Size |
#Plates at 300uL/well
(28.8mL/plate)
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#Plates at 400uL/well
(38.4mL/plate) |
Price |
| EBB300-1 |
100 |
3 |
2 |
€45.00 |
| EBB300-2 |
500
|
16 |
12 |
€140.00 |
| EBB300-3 |
1,000 |
33 |
25 |
€270.00 |
| EBB300-4 |
10,000 |
330 |
250 |
€2300.00 |
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