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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
Marienbongard 20
52074 Aachen,
Germany
Tel 0241 56 00
99 68
Fax 0241 56 00 47 88
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 58 760 77
08
Fax: 00 32 16 50 90
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GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
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Fax 02 36 00 65
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Česká republika
Praha
+420246019719
Danmark
+4569918806
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+358942419041
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+302111768494
Ireland Dublin
+35316526556
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+35220880274
Magyarország
Budapest
+3619980547
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+31208080893
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+4721031366
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+43720880899
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+46852503438
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+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
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Carboxyfluorescein Caspase
Detection Kits ™
Apoptosis detection
and in situ labeling of active caspase in live
cells.
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Key Benefits: |
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Non-cytotoxic assay arrests further
apoptotic activity via caspase
inhibition.
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Cell permeablity permits direct
visualization of cytosolic apoptotic
events.
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Apoptotic cell population does not
diminish over time.
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Add reagent directly to cells. No
special buffer or media needed. No
preparation of cell lysates required.
Simple wash procedure.
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Works in diverse cell lines: human,
rodent, Drosophila.
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Can be performed in conjunction with
Annexin staining, TUNEL, antibody
staining, or with other APO LOGIX
reagents on the same population of
cells.
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Permits high through-put screening.
Protocol can be adapted for ex vivo as
well as in situ experiments.
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Works with fluorescence microscopy,
96-well fluorescence plate readers, or
flow cytometry.
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Yields both quantitative and qualitative
results. Gives strong signal with little
background noise.
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Mark activity across the range of
caspase proteins. Poly-caspase and
caspase-specific assays to target
caspases 1, 2, 3, 6, 8, 9, or 10 are
available.
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Applications - Flow Cytometry,
Fluorescence Plate Reader, Fluorescent
Microscope
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Assay Principle |
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APO LOGIX Carboxyfluoroscein Caspase
Detection Kits label active caspases in
living cells undergoing apoptosis. Cell
Technology’s probes utilize
carboxyfluorescein(FAM)-labeled peptide
fluoromethyl ketone (FMK) caspase inhibitors
(FAM-peptide-FMK). These FAM-peptide-FMK
compounds are both cell permeable and non-cytotoxic
during the course of the assay and thus
allow the detection of active caspases in
living cell systems.
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| Kit contents |
- Lyophilized FAM labeled peptide
inhibitor
- 10X Wash Buffer
- 10X Fixative
- Propidium Iodide
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The following kits are available: |
Product
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Catalog No.
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Size
(No. of Tests) |
Price (Eur)
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FAM-VAD-FMK Poly caspase detection kit |
FAM100-1 |
25 |
€145 |
FAM100-2
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100 |
€425 |
FAM-DEVD-FMK Caspase 3 detection kit
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FAM200-1 |
25 |
€145 |
FAM200-2
|
100 |
€425 |
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FAM-LETD-FMK Caspase 8 detection kit |
FAM300-1 |
25 |
€155 |
FAM300-2
|
100 |
€445 |
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FAM-LEHD-FMK Caspase 9 detection kit |
FAM400-1 |
25 |
€145 |
FAM400-2
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100 |
€425 |
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FAM-VEID-FMK Caspase 6 detection kit |
FAM500-1 |
25 |
€195 |
FAM500-2
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100 |
€495 |
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FAM-YVAD-FMK Caspase 1 detection kit |
FAM600-1 |
25 |
€155 |
FAM600-2
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100 |
€445 |
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FAM-VDVAD-FMK Caspase 2 detection kit |
FAM700-1 |
25 |
€195 |
FAM700-2
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100 |
€495 |
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FAM-AEVD-FMK Caspase 10 detection kit |
FAM800-1 |
25 |
€195 |
FAM800-2
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100 |
€495 |
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