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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
Marienbongard 20
52074 Aachen,
Germany
Tel 0241 56 00
99 68
Fax 0241 56 00 47 88
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 58 760 77
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Fax: 00 32 16 50 90
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GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
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Fax 02 36 00 65
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Česká republika
Praha
+420246019719
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+4569918806
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+358942419041
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+35316526556
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+35220880274
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+3619980547
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+31208080893
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+4721031366
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+43720880899
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+46852503438
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+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
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APO LOGIX Sulforhodamine Caspase Detection
Kit ™
Apoptosis detection and in situ
labeling of active caspase in live cells.
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| Key Benefits:
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Non-cytotoxic assay arrests further apoptotic activity
via caspase inhibition.
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Cell permeablity permits direct visualization of
cytosolic apoptotic events.
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Apoptotic cell population does not diminish over time.
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Add reagent directly to cells. No special buffer or
media needed. No preparation of cell lysates required.
Simple wash procedure.
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Works in diverse cell lines: human, rodent, Drosophila.
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Can be performed in conjunction with Annexin staining,
TUNEL, antibody staining, or with other APO LOGIX
reagents on the same population of cells.
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Permits high through-put screening. Protocol can be
adapted for ex vivo as well as in situ experiments.
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Applications - Works with fluorescence
microscope, 96-well fluorescence plate readers
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Yields both quantitative and qualitative results. Gives
strong signal with little background noise.
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| Assay Principle |
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APO LOGIX SR kits contain a generic sulforhodamine labeled
caspase inhibitor (sulforhodamine-peptide-fluoromethyl
ketone). This reagent is cell permeable and is used on whole
cells to detect apoptosis. Apoptotic cells are detected by a
fluorescence plate reader or fluorescence microscope using
an excitation source at 550nm and measuring emission at
595nm. The assay takes about 1 hr to complete
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| Kit contents |
- Lyophilized SR-VAD-FMK
- 10X Wash Buffer
- 10X fixative
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| The following
kits are available: |
Product
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Catalog No.
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Size
(No. of Tests) |
Price (Eur) |
| SR-VAD-FMK Poly
caspase detection kit |
SR100-1 |
25 |
€195 |
SR100-2
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100 |
€525 |
SR-DEVD-FMK Caspase 3
detection kit
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SR200-1 |
25 |
€195 |
SR200-2
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100 |
$525 |
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