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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
Marienbongard 20
52074 Aachen,
Germany
Tel 0241 56 00
99 68
Fax 0241 56 00 47 88
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 58 760 77
08
Fax: 00 32 16 50 90
45
GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
93
Fax 02 36 00 65
94
Česká republika
Praha
+420246019719
Danmark
+4569918806
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+358942419041
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+302111768494
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+35316526556
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+35220880274
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Budapest
+3619980547
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+31208080893
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Oslo
+4721031366
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+43720880899
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+46852503438
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+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
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Conjugate Diluents |
| Improves Assays:
|
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Increases the Sensitivity of the Assay.
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Decreases Backgrounds.
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Stabilizes Newly Conjugated Proteins.
Also reconstitutes lyophilized conjugates and
diluents concentrates conjugates.
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Prolongs the Shelf-Life of HRP-conjugated
proteins and enhancing their utility in ELISA
applications.
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Extends the Useful Titer of the
conjugate.
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Conserves Valuable Reagents. By
promoting a higher specific signal, less
conjugate may be needed to generate a signal
when a stabilizing conjugate diluent is used.
-
Lengthens the Shelf-Life of
conjugated antibodies for future use. With a
longer shelf-life, conjugates may be prepared in
batches for use in future plate coating runs.
Using conjugates from the lot generally reduces
inter-assay variability and increases
consistency.
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| How it
Works: |
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Conjugate Diluents stabilize newly conjugated
proteins, reconstitute yophilized conjugates, and
dilute concentrated conjugates. Cell Technology's
conjugate diluents stabilize the immunoglobulin and
HRP components of the conjugate - protein complex.
All conjugate diluents are available at 1x and 5x
concentrations. When stored unopened and protected
from light, a shelf-life of 18 months can be
expected at 2°-8°C.
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| Directions: |
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To stabilize newly conjugate proteins, add 1 part of
the appropriate 5x conjugate diluent (that has not
been diluted to 1x) to 4 parts of the new conjugate
solution.
To reconstitute lyophilized conjugates, simply
pipette a known amount of a 1x conjugate diluent
into the vial. The liquid conjugate is now ready to
be used directly, or further diluted to the required
concentration.
To dilute concentrated conjugates for use in an
assay, pipette a known amount of the conjugate stock
into a 1x conjugate diluent.
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ECD100 Monoclonal-
Polyclonal Conjugate Diluent & Stabilizer |
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Monoclonal-Polyclonal Conjugate Diluent & Stabilizer
is a unique formulation specifically designed for
use in mouse monoclonal capture and polyclonal-HRP
sandwich ELISA formats. This diluent contains mouse
proteins which suppress nonspecific binding of the
polyclonal-HRP conjugate to the mouse
immunoglobulins adsorbed onto the plate. These
protein additives also provide additional structural
stability to the conjugate protein complex. Other
additives contained in ECD100 serve to extend the
useful titer of the conjugate by stabilizing both
the immunoglobulin and HRP components of the
conjugate. Antimicrobial agents have also been added
to retard bacterial growth. When stored unopened and
protected from light, a shelf-life of 18 months can
be expected at 2°-8°C.
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| 5x Monoclonal -
Polyclonal Diluent & Stabilizer (ECD105) |
Catalog Number
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final volume at 1x
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Bottle size (mL)
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Plates at 100uL/well 1x
(9.6mL/plate) |
Plates at 200uL/well
(19.2mL/plate) |
Price |
| ECD105-1 |
500 mL |
100 |
50 |
25 |
€220.00 |
| ECD105-2 |
2.5L
|
500 |
255 |
126 |
€695.00 |
| ECD105-3 |
5L |
1000 |
515 |
255 |
€1175.00 |
| ECD105-4 |
50L |
10000 |
5200 |
2600 |
€10900.00 |
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| ECD200
Antigen-Down Conjugate Diluent & Stabilizer |
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Antigen-Down Conjugate Diluent & Stabilizer is a
unique formulation specifically designed for use
with anti-IgG-HRP conjugates. This diluent contains
proprietary non-mammalian protein additives which
will not interfere with anti-IgG-HRP conjugates used
in antigen-down and sandwich ELISA formats. These
additives also provide additional structural
stability to the IgG-HRP conjugate protein complex.
CD2 will also inhibit conjugate binding to
nonspecific serum proteins bound to the plate, thus
reducing conjugate bridging on the plate surface and
lowering background noise. Antimicrobial agents have
been added to retard bacterial growth. When stored
unopened and protected from light, a shelf-life of
18 months can be expected at 2°-8°C.
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| 5x
Antigen- Down Conjugate Diluent & Stabilizer
(ECD205) |
Catalog Number
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Final volume at 1x
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Bottle size (mL)
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Plates at 100uL/well 1x
(9.6mL/plate)
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Plates at 200uL/well 1x
(19.2mL/plate) |
Price |
| ECD205-1 |
500mL
|
100
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50 |
25 |
€220.00 |
ECD205-2
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2.5L |
500 |
255 |
126
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€695.00 |
ECD205-3
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5L |
1000 |
512 |
255 |
€1175.00 |
| ECD205-4 |
50L |
10000 |
5200 |
2600 |
€10900.00 |
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| ECD300
Polyclonal-Polyclonal Conjugate Diluent & Stabilizer |
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Polyclonal-Polyclonal Conjugate Diluent & Stabilizer
is a unique formulation specifically designed for
use in polyclonal capture and polyclonal-HRP
sandwich ELISA formats. Proprietary additives were
specifically selected to provide optimal assay
performance when using rabbit, goat, or rabbit and
goat polyclonal antibodies in a sandwich ELISA
format. ECD300 contains a uniquely processed form of
rabbit and goat IgG to suppress nonspecific binding
of the polyclonal-HRP conjugates to the plate
surface. To reduce nonspecific binding interactions,
a detergent is also included. Antimicrobia agents
have been added to retard bacterial growth. When
stored unopened and protected from light, a
shelf-life of 18 months can be expected at 2°-8°C.
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| 5x
Polyclonal - Polyclonal Conjugate Diluent &
Stabilizer (x CD3) |
Catalog Number
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Final volume at 1x
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Bottle size (mL) |
Plates at 100uL/well 1x
(9.6mL/plate)
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Plate at 200uL/well 1x
(19.2mL/plate) |
Price |
ECD305-1
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500mL
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100 |
50 |
25 |
€490.00 |
ECD305-2
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2.5L |
500 |
255 |
126 |
€1995.00 |
| ECD305-3 |
5L |
1000 |
515 |
255 |
€3700.00 |
| ECD305-4 |
50L |
10000
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5200 |
2600 |
€34500.00 |
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| ECD400 Monoclonal
- Goat Polyclonal Conjugate Diluent & Stabilizer |
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This diluent is a unique formulation specifically
designed for use in monoclonal capture and goat
polyclonal-HRP sandwich ELISA formats. Additives
were carefully selected to specifically address the
stability and nonspecific binding issues associated
with this type of ELISA format. Goat proteins are
included to reduce any nonspecific interactions
between the monoclonal capture and goat polyclonal-HRP
antibodies. CD4 also contains mouse IgG to
specifically suppress nonspecific bridging of the
goat polyclonal-HRP conjugate with the mouse capture
IgG on the plate surface. To further reduce
nonspecific binding of the conjugate to the plate
surface, adetergent is also included. Antimicrobial
agents have been added to retard bacterial growth.
When stored unopened and protected from light, a
shelf-life of 18 months can be expected at 2°-8°C.
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| 5x
Monoclonal - Goat Polyclonal HRP Conjugate
Diluent & Stabilizer (ECD405) |
Catalog Number
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Final volume at 1x
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Bottle Size (mL)
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Plates at 100uL/well 1x
(9.6mL/plate)
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Plates at 200uL/well 1x
(19.2mL/plate)
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Price |
ECD405-1
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500ml
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100 |
50
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25 |
€295.00 |
| ECD405-2 |
2.5L |
500 |
255 |
126 |
€895.00 |
| ECD405-3 |
5L |
1000 |
515
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255
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€1495.00 |
| ECD405-4 |
50L |
10000 |
5200 |
2600 |
€12995.00 |
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| ECD600 Conjugate
Stock Stabilizing Reagent |
Conjugate Stock Stabilizing Reagent contains a BSA-based
mammalian protein additive to stabilize conjugated
proteins, and magnesium and calcium salts to
stabilize the catalytic site of the HRP porphyrin
ring structure. When diluted to 1x, ECD600 is an
excellent HRP conjugate diluent and stabilizer base,
to which additional components can be added to for
use in your specific assay.
At 5x, ECD600 is an excellent diluent for
stabilizing newly conjugated proteins. This allows
the liquid conjugate stock to be stored, and then
later diluted to make a working conjugate for use in
the assay. Simply add 1 part of 5x CD6 (that has not
been diluted to 1x) to 4 parts of the new conjugate
solution. For example, if you just eluted 20 mL of
an antibody-HRP conjugate off a column, simply add 5
mL of 5x CD6 to the conjugate solution. If the
conjugate was initially at 10 mg/mL, the stabilized
conjugate will now be at 8 mg/mL in 25mL diluent
(it's been diluted by 20%).
Antimicrobial agents have also been added to ECD6
retard bacterial growth. When stored unopened and
protected from light, a shelf-life of 18 months can
be expected at 2°-8°C.
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| 5x
Conjugate Stock Stabilizing Reagent (ECD605)
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Catalog Number
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Final volume at 1x
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Bottle size (mL)
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#Plates at 100uL/well 1x
(9.6mL/plate)
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#Plates at 200uL/well 1x
(19.2mL/plate)
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Price |
ECD605-1
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500mL
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100 |
50
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25 |
€195.00 |
| ECD605-2 |
2.5L |
500 |
255
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125 |
€595.00 |
| ECD605-3 |
5L |
1000 |
515 |
255 |
€1095.00 |
| ECD605-4 |
50L |
10000 |
5200 |
2600 |
€8895.00 |
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