Fluorescent Thiol Detection Kit

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN

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During the normal course of metabolism, oxygen is partly reduced as electrons leak out of the electron transport chain during respiration. These partially reduced oxygen species (ROS) can react with organic substances through non-catalytic means. Furthermore, ROS can be generated via endogenous enzyme systems like plasma NADPH oxidase, cytoplasmic xanthine oxidase and organelle sources e.g., cytochrome P-450. ROS have been implicated in regulating diverse cellular functions including proliferation, defense against pathogens, intra-cellular signaling, transcriptional activation and apoptosis. Elevation of ROS beyond the buffering capacity of the cell can lead to oxidative stress. Elevated ROS levels can lead to damage of DNA/RNA, proteins and lipids which may lead to apoptosis. Cells have developed several mechanisms to counter act elevated ROS levels such as a thiol reducing buffer composed of cellular thiol levels (glutathione and thioredoxion) for the maintenance of the reduction-oxidation (redox) state of the cell, and enzymes to remove ROS (catalase, superoxide dismutase and glutathione peroxidase) (1-2).