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KOMABIOTECH
301, Gayang Technotown, #1487 Gayang 3 dong, Gangseo-gu
Seoul 157-793, KOREA

Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464

SCETI K.K
BIOSCIENCE Export                                   DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN 

EY Laboratories, Inc. Headquarters
107 N. Amphlett Blvd
San Mateo, CA. 94401 USA

EXBIO Praha, a.s.
Nad Safinou II 366
252 42  Vestec
Czech Republic


 Sacace Biotechnologies S.r.l.
Via Scalabrini, 44
22100 Como Italy
 

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany Marienbongard 20

52074 Aachen, Germany

Tel  0241 56 00 99 68                    Fax 0241 56 00 47 88 

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 58 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

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+4569918806 

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+43720880899 

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+46852503438 

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+41435006251 

 

Northern America 

Canada Montreal
+15149077481 

US New York
+17185132983

 

Other Countries redcoon België
 0032 (0)16 41 44 07

 
Fluoro H202™
Fluorescent Hydrogen peroxide/Peroxidase Detection Kit
 
Key Benefits:
  • Quick 10 minute assay
  • Can monitor multiple time points to follow kinetics.
  • Dual mode, can detect H202 or peroxidase activity.
  • One-step, no wash assay.
  • Adaptable for High Throughput format
  • Non-destructive cell based assay allows monitoring of additional parameter
  • Applications - Fluorescent Plate Reader
Assay Principle
The Fluoro H202 detection kit utilizes a non-fluorescent detection reagent, to detect H202. H202 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H202 release from cells or enzyme coupled reactions (1-7)
Reaction:
                                                            peroxidase
H202 + detection reagent (non-fluorescent) --------->   Resorufin
                                                                            (fluorescent)
    Excitation: 530-571nm                                                        Emmission: 590-600nm
The following kits are available:
Catalog 
 		 Size
 		 Price (Eur)
FLOH 100-3
 		 500 Tests €215
Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H202 Kit.