Apo 3/7 HTS™ High Throughput Screen for Caspase 3/7
detection
Key Benefits:
Homogenous assay for active caspase 3/7.
Breakthrough in cell lysis buffer and preservation of caspase
activity.
Results in a No wash one-step assay
No need to wash out media from cell samples, just add the
reagent directly to your experimental samples.
Easy to Use: No need to make cell lysates or run western blots.
Works with suspension and adherent cells.
Applications - Standard Fluorescence Plate Reader
Assay
Principle
Cell Technology’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110
peptide substrate for caspase 3/7 detection. The absorption and
emission properties of the R110 dye are suppressed when attached to
the z-DEVD peptide sequence. When R110 is cleaved away, by active
caspase3/7, form the quenching DEVD sequence, the free dye excites
at 488nm and emits at 515-530 nm.. As a result of a novel and
proprietary Lysis Buffer System, the Apo 3/7 HTS Assay is a
homogenous platform that can be utilized for high throughput
fluorescence plate reader applications. The reagent is directly
added to the samples thus eliminating any wash steps.
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