JC-1 Mitochondrial Membrane
Potential Detection Kit In situ detection of
apoptosis and mitochondrial membrane potential.
Key Benefits:
Cell permeability allow direct
measurement of apoptosis and mitochondrial
potential in live cells.
Applications - Cells can be
analyzed by flow cytometry, 96 well plate
reader or fluorescence microscope.
Incubate for 15 minutes, wash and
measure.
Add this reagent directly to live cells
in your media of choice.
Assay Principle
Detection of the mitochondrial permeability
transition event provides an early
indication of the initiation of cellular
apoptosis. This process is typically defined
as a collapse in the electrochemical
gradient across the mitochondrial membrane,
as measured by the change in the membrane
potential (YD). Loss of mitochondrial YD is
indicative of apoptosis and can be detected
by a unique fluorescent cationic dye,
5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-
benzamidazolocarbocyanin iodide, commonly
known as JC-1. This dye has been
incorporated into the user-friendly kit for
the simple and reproducible detection of the
membrane potential (Y)D event in apoptotic
cells. The kit has been formatted for use on
flow cytometers and fluorometric plate
readers.
Kit contents:
Lyophilized JC-1 Dye
10X Assay buffer
The following kit is available:
Description
Catalog No.
Size
Price
APO LOGIX JC-1 Kit
JC100
100 Tests
€245
(A)
(B)
Jurkat cells were cultured with DMSO
(A) or staurosporine (B) for 2
hours. Cell were then stained with
JC-1 Mitochondrial Membrane
Potential Detection Kit for 15
minutes and analyzed by flow
cytometry.
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