Mito Flow Mitochondria Membrane Potential
detection for Flow Cytometry
Key Benefits:
Cell Permeable, Easy One Color Assay for Flow Cytometry
Can be used with both suspension and monolayer adherent
cell lines.
1 Step, No Wash, Adaptable for High Throughput format
Compatible with other antibodies or stains. For example
flourescent protein expression vectors.
Applications - Flow Cytometer
Assay Principle:
Cell Technology’s Mito Flow assay utilizes a cationic dye to
visualize mitochondrial membrane potential (5-7). The Mito
Flow reagent is a cell permeable cationic dye that has a
strong fluorescent signal and exhibits low membrane
potential independent (non specific) binding and toxicity.
In healthy cells the Mito Flow reagent is accumulated by the
mitochondria in proportion to the DeltaPsi (membrane
potential). In most cell lines, accumulation of the Mito
Flow reagent in the mitochondria results in a higher
fluorescence intensity. In apoptotic cells, where the
mitochondrial membrane potential is compromised, the Mito
Flow reagent does not get accumulated in the mitochondria
and these cells exhibit a lower fluorescence signal.
Kit contents:
Vial of Mito Flow Dye Part#4004
Bottle of 10X dilution buffer Part#3004
The following
kits are available:
Catalog
Size
Price
Flo200-2
100 Tests
€245
Flo200-3
500 Tests
€475
(A)
(B)
Figure 1. Jurkat cells were stimulated with
Staurosporine (B) or DMSO (A) for 3 hours. Cells
were then stained by Mito Flow and analyzed by Flow
Cytometry
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